Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by SHIMADA, H.
Right arrow Articles by ORII, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SHIMADA, H.
Right arrow Articles by ORII, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1976, Vol. 80, No. 1 135-140
© 1976 Japanese Biochemical Society

research-article

Oxidation-Reduction Behavior of the Heme c and Heme d Moieties of Pseudomonas aeruginosa Nitrite Reductase and the Formation of an Oxygenated Intermediate at Heme d1

Hideo SHIMADA and Yutaka ORII

Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560

Dithionite reduced the heme c moiety of Pseudomonas nitrite reductase almost instantaneously, whereas the spectral change of heme d proceeded in two steps, requiring at least 15 min for completion. The final spectrum coincided well with that obtained by anaerobic reduction with ascorbate, during which a quasi oxidation-reduction equilibrium was established between the two heme groups. The difference in apparent redox potential was calculated to be 24 mV, heme d being more negative.

When the enzyme was supplemented with a reductant and molecular oxygen, an oxygenated intermediate appeared at the heme d moiety.

1 This study was supported in part by a grant from the Ministry of Education, Science and Culture, of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. Nurizzo, F. Cutruzzola, M. Arese, D. Bourgeois, M. Brunori, C. Cambillau, and M. Tegoni
Does the Reduction of c Heme Trigger the Conformational Change of Crystalline Nitrite Reductase?
J. Biol. Chem., May 21, 1999; 274(21): 14997 - 15004.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.