Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by SUGAHARA, K.
Right arrow Articles by YAMASHINA, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SUGAHARA, K.
Right arrow Articles by YAMASHINA, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1976, Vol. 80, No. 2 195-201
© 1976 Japanese Biochemical Society

research-article

Characterization of One Neutral and Two Acidic Glycoasparagines Isolated from the Urine of Patients with Aspartylglycosylaminuria (AGU)1

Kazuyuki SUGAHARA, Susumu FUNAKOSHI, Ikuo FUNAKOSHI, Pertti AULA* and Ikuo YAMASHINA2

Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University Sakyo-ku, Kyoto, Kyoto 606
*Department of Pathology, University of Helsinki Helsinki, Finland

2 Correspondence should be addressed to I. Yamashina.

One neutral and two acidic glycoasparagines were isolated from the urine of patients with aspartylglycosylaminuria (AGU). The neutral one was identified as ß-Gal-(1{uparrow}4)-ß-GICNAc-Asn. The acidic ones were composed of 1 mole of sialic acid and 2 moles each of galactose and N-acetylglucosamine, attached to asparagine, and were isomeric with respect to the position of sialic acid attachment since they produced the same glycoasparagine on incubation with the neuraminidase [EC 3.2.1.18 [EC] ] from Clostridium perfringens. The structure of the resulting sialic acid-free glycoasparagine was determined to be ß-Gal-ß-GIcNAc-ß-Gal-(1{uparrow}4)-B-GlcNAc-Asn based mainly on the results of sequential enzymatic degradations.

1 This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.