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J. Biochem, 1976, Vol. 80, No. 2 351-359
© 1976 Japanese Biochemical Society

research-article

Studies on Triglyceride Lipases from Rat Adipose Tissue

Sueo MATSUMURA, Takanori MATSUDA1, Masafumi MATSUO2, Akira KUMON3, Katsuichiro SUDO4 and Yasutomi NISHIZUKA

Department of Biochemistry, Kobe University School of Medicine Ikuta-ku, Kobe, Hyogo 650

A new assay procedure for triglyceride lipase [EC 3.1.1.3 [EC] ] was developed in which radioactive triolein was dissolved in ethanol and directly added to the reaction mixture in the absence of serum and albumin. In the rat adipose tissue there appeared to be a triglyceride lipase measurable with this assay in addition to the two previously defined lipases, lipoprotein lipase [EC 3.1.1.34 [EC] ] and hormone-sensitive lipase. The enzyme was active in the absence of serum and was strongly inhibited by albumin. The molecular weight was estimated to be about 42,000. Adenosine 3', 5'-monophosphate-dependent protein kinase [EC 2.7.1.37 [EC] ] was unable to activate the enzyme. The three species of lipases mentioned above behaved differently upon chromatography on a Sepharose 4B column, and were distinguishable from each other in their physical and kinetic properties. The physiological roles of the new species of lipase remain to be explored.

1 Present address: Department of Surgery, First Division, Kobe University School of Medicine, Kobe, Japan.

2 Present address: Department of Pediatrics, Kobe University School of Medicine, Kobe, Japan.

3 Present address: Psychiatric Research Institute of Tokyo, Division of Neurochemistry, Tokyo, Japan

4 Present address: Biological Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Osaka, Japan.


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