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J. Biochem, 1976, Vol. 80, No. 2 367-370
© 1976 Japanese Biochemical Society

research-article

Effective Method for Activity Assay of Lipase from Chromobacterium viscosum1

Yosifumi HORIUTI, Hajime KOGA and Sinobu GOCHO2

Research Laboratory, Toyo Jozo Co., Ltd., Mifuku Ohito-cho, Tagata-gun, Shizuoka 410-23

A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3 [EC] ] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10–16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase activity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate.

1 Reported at the annual meeting of the Japanese Conference on the Biochemistry of Lipids held at Matsumoto-shi (1974).

2 Present address: Research Laboratory, Hasegawa Koryo Co., Ltd., Kariyado, Nakahara-ku, Kawasaki-shi, Kanagawa 211.


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