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J. Biochem, 1976, Vol. 80, No. 2 371-377
© 1976 Japanese Biochemical Society

research-article

A New Bile Acid Conjugate, Ciliatocholic Acid, from Bovine Gall Bladder Bile1

Masato TAMARI, Masafumi OGAWA2 and Masao KAMETAKA

Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113

This study was carried out to investigate the occurrence of ciliatocholic acid in bovine gall bladder bile.

Ciliatocholic acid was synthesized according to the method described by Bergström and Norman for the synthesis of taurocholic acid. Elemental analysis, melting point, and the infrared spectrum of this substance were determined.

An isolation procedure for ciliatocholic acid was established by stepwise elution with an HCl-ethanol solvent system using a Dowex-1 anion exchange resin column chromatographic technique. Ciliatocholic acid amounting to 158 µg (as ciliatine) per 100 ml of gall bladder bile was found in the fraction eluted with 0.01 N HCl in 50% ethanol.

This compound was purified by preparative thin-layer chromatography and confirmed to be ciliatocholic acid from the hydrolytic stability, phosphorus determination, and chromatographic behavior. Thus, bovine gall bladder bile contains a small amount of ciliatocholic acid.

1 This report was presented at the General Meeting of the Agricultural Chemical Society of Japan, held in Sapporo, July 25, 1975.

2 Present address: Institute for Plant Virus Research, Aoba-cho, Chiba-shi, Chiba 959.


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