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J. Biochem, 1976, Vol. 80, No. 2 413-415
© 1976 Japanese Biochemical Society

other

Isolation of Myosin from Starfish Sperm Heads

Issei MABUCHI

Department of Biology, College of General Education, University of Tokyo Komaba, Meguro-ku, Tokyo 153

Myosin was extracted and partially purified from the head portion of spermatozoa of the starfish, Asterias amurensis. The sperm myosin showed a specific Ca2+-activated ATPase [EC 3.6.1.3 [EC] ] activity of 0.2 {alpha}moles P1/min/mg at high ionic strength and pH 6.5. It resembled egg myosin in forming thick filaments, becoming attached to actin filaments, subunit composition, and serological properties.


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