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J. Biochem, 1977, Vol. 81, No. 4 1063-1070
© 1977 Japanese Biochemical Society

research-article

Chemical Properties of the Polysaccharides Associated with Acid Protease of Aspergillus oryzae Grown on Solid Bran Media

Yoshio TSUJITA and Akira ENDO

Fermentation Research Laboratories, Sankyo Co., Ltd. Hiromachi, Shmagawa-ku, Tokyo 140

The chemical and physical properties of the carbohydrate moiety of acid protease A1 [EC 2.4.23.6 [EC] ], one of the two acid proteases produced by Aspergilius oryzae grown on solid branmedia, have been studied. The enzyme was separated into two active components (A1a and A1b) by isoelectric focusing; both contained approximately 50 % carbohydrate. The carbohydrate portion of the major component A1a was composed of galactose, glucose, and mannose, but the minor component A1b contained glucose as the predominant carbohydrate, with smaller amounts of galactose, fructose, xylose and mannose.

The absence of a covalent linkage between the polysaccharide and the protein in acid protease A1 was indicated, although these two moieties were tightly associated with each other. This conclusion was based on the following observations: (a) the polysaccharides obtained after proteolytic digestion contained no detectable amino acids; (b) no hexosamines were detected in acid protease A1; (c) the polysaccharides were not separable from the protein by a variety of procedures, including treatment with cold 15% trichloroacetic acid, 8 M urea, 6 M guanidine hydrochloride, and 0.1 N NaOH (at 25°C for 18 h). However, when treated with 50% acetic acid at 37°C for 48 h, the polysaccharides were completely dissociated from the protein; and (d) of several glycoproteins tested, containing an N-glycosyl or 0-glycosyl linkage between the protein and carbohydrate moieties, none liberated carbohydrate residues upon treatment with 50% acetic acid.


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