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J. Biochem, 1977, Vol. 81, No. 4 835-842
© 1977 Japanese Biochemical Society

research-article

Production of Copper Coproporphyrin III by Bacillus cereus

I. Purification and Identification of Copper Coproporphyrin III

Takako YAMADA-ANKEI*,1, Hidekazu IWASAKI** and Takeshi MORI**

*Botanical Institute, Faculty of Science, Hiroshima University Hiroshima, Hiroshima 730
**Botanical Institute, Faculty of Science, Nagoya University Chikusa-ku, Nagoya, Aichi 464

Bacillus cereus strains 2 and T did not form spores and accumulated a large amount of purple pigment inside the cells, when cultured in a yeast extract-ammonium salt medium with excess glucose. The pigment was extracted and crystallized as the ethyl ester. It was identified as copper coproporphyrin III.

1Present address: Laboratory for Plant Ecological Studies, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto, Kyoto 606.


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