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J. Biochem, 1977, Vol. 81, No. 4 891-898
© 1977 Japanese Biochemical Society

research-article

Fluorescent Probes for Antibody Active Sites

II. Further Studies on Two Groups of Anti-MANS Antibodies with Significantly Different Effects on MANSamide Fluorescence Produced by a Single Rabbit

Masahiko ONODERA and Hiroyuki SHIOKAWA

Institute of Immunological Science, Hokkaido University Kita-ku, Sapporo, Hokkaido 060

As described previously, a single rabbit (No. 13) produced two groups of anti-MANS antibodies with significantly different effects on MANSamide fluorescence. These groups, anti-MANS antibody (415 nm) and anti-MANS antibody (465 nm), had emission maxima at 415 nm and 465 nm, respectively.

This paper deals with the immunochemical properties of the two groups of antibodies

  1. The results of fluorometric titration of these groups with MANSamide indicated that anti-MANS antibody (465 nm) had a higher affinity for MANSamide than did anti-MANS antibody (415 nm).
  2. More than 70% of anti-MANS antibody 415 nm) was adsorbed on Sepharose covalently coupled with bovine serum albumin, which was used as a carrier during immunization, while a half of anti-MANS antibody (465 nm) was adsorbed.
  3. Only one of the groups, anti-MANS antibody (465 nm), had precipitating activity toward MANS-rabbit serum albumin used as a test antigen.


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