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J. Biochem, 1977, Vol. 81, No. 5 1181-1186
© 1977 Japanese Biochemical Society

research-article

The Structure of the Branching Point between Acidic Polysaccharide kind Peptidoglycan in Micrococcus lysodeikticus Cell Wall

Sumihiro HASE and Yoshio MATSUSHIMA

Department of Chemistry, Osaka University College of Science Toyonaka, Osaka 560

An acidic polysaccharide fraction composed of glucose and N-acetylmannosaminuronic acid with a small portion of peptidoglycan was isolated by enzymic digestion and subsequent ECTEOLA-cellulose chromatography from the cell walls of Micrococcus Iysodeikticus. On mild acid treatment, the fraction became Morgan-Elson positive and formed the Morgan-Elson chromogen on heating with phosphate buffer (pH 7). The product of mild acid treatment released inorganic phosphate on treatment with phosphomonoesterase. After gel-chromatography on Sephadex G-25 and DE-32, the acidic polysaccharide fraction contained less glucosamine than muramic acid. By reduction of this fraction with borohydride, a part of the glucosamine was converted into glucosaminitol. Based on these results, it is suggested that the acidic polysaccharide is linked to glucosamine by a (1–3) linkage, which is linked to the 6 position of a muramic acid residue by a phosphodiester inkage.


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