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J. Biochem, 1977, Vol. 81, No. 5 1187-1192
© 1977 Japanese Biochemical Society

research-article

Hydrolysis of Phenyl ß-Maltoside Catalyzed by Saccharifying {alpha}-Amylase from Bacillus subtilis1

Kiyoshi ISHIKURA2, Yasunori NITTA and Takehiko WATANABE

Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka Prefecture Sakai, Osaka 591

  1. The hydrolytic reaction of phenyl ß-maltoside catalyzed by saccharifying {alpha}-amylase [EC 3.2.1.1 [EC] [EC] ] of Bacillus subtilis was studied at 25°C and pH 5.4, by measuring the total reducing power and the amount of phenol liberated, and by thin layer chromatography.
  2. The enzyme hydrolyzed phenyl ß-maltoside at the glucosidic linkage between the two glucose residues to form D-glucose and phenyl ß-D-glucoside. Besides these products, maltose, maltotriose, and phenyl ß-maltotrioside were also observed as reaction products. The identification of phenyl ß-maltotrioside is described in detail. The formation of these products was attributed to the transglycosylation reaction of the enzyme.

The time course of reaction as followed by reducing power measurement showed an induction period of several minutes.

1A part of this study was presented at the Annual Meeting of the Agricultural Chemical Society of Japan, Tokyo, April 1974.

2Present address: Environmental Pollution Control Section, Department of Hygiene, Nara Prefecture Government, Noborióji-cho, Nara, Nara 630.


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