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J. Biochem, 1977, Vol. 81, No. 5 1203-1208
© 1977 Japanese Biochemical Society

research-article

Isolation of Glycopeptides from the Lectin-stimulated Human Peripheral Lymphocyte Cell Surface1

Mariko SAITO, Satoshi TOYOSHIMA and Toshiaki OSAWA

Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo Bunkyo-ku, Tokyo 113

Three radioactive glycopeptides were isolated from human peripheral lymphocytes stimulated with Wistaria floribunda mitogen in the presence of D- [14C] glucosamine hydrochloride by mild trypsin digestion followed by gel filtration and preparative high-voltage paper electrophoresis. The carbohydrate compositions of these glycopeptides suggest that one has a sugar chain of the type found in serum glycoproteins, consisting of sialic acid, galactose, N-acetylglucosamine, mannose, and fucose in a molar ratio of 2:2:4:2:1, and the other two have sugar chains like those of mucins, consisting of sialic acid, galactose, and N-acetylgalactosamine in a molar ratio of I or 2:1:1. The results of enzymic degradation with purified glycosidases indicate that these sugar chains are similar in structure to their counterparts in human erythrocyte membranes.

1This work was supported by a research grant from the Ministry of Education, Science and Culture of Japan.


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