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J. Biochem, 1977, Vol. 81, No. 5 1227-1230
© 1977 Japanese Biochemical Society

research-article

A Method for Incorporating Labeled Lecithin into Serum Low Density Lipoproteins in vitro

Shinji YOKOYAMA*, Masaharu TAKAYAMA** and Yasuo AKANUMA**

*The Institute for Adult Diseases, Asahi Life Foundation Nishishinjuku, Shinjuku-ku, Tokyo 160
**The Third Department of Internal Medicine, Faculty of Medicine, the University of Tokyo Hongo, Bunkyo-ku, Tokyo 113

A sonicated dispersion of [14C]lecithin was incubated with high density lipoproteins (HDL) coupled to Sepharose. After washing the gels thoroughly with a buffer, the gels were incubated with low density lipoproteins (LDL); [14C]lecithin was transferred from the sonicated dispersion via HDL-Sepharose to the LDL.

The LDL fraction thus prepared showed no contamination with lecithin dispersion or HDL. The lecithin: cholesterol acyltransferase (LCAT) reaction could be completely inhibited during preparation, and the net recovery of radioactivity in LDL was 16% of that of the original lecithin dispersion.

The [14C]lecithin in the washed HDL-Sepharose was shown to be a substrate of the LCAT reaction in vitro.


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