Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by SUZUKI, N.
Right arrow Articles by SHUKUYA, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SUZUKI, N.
Right arrow Articles by SHUKUYA, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1977, Vol. 81, No. 5 1231-1236
© 1977 Japanese Biochemical Society

research-article

Isolation and Partial Characterization of the Serum Low Density Lipoprotein from Bullfrog, Rana catesbeiana

Norio SUZUKI*, Shizue KAWASHIMA*, Sumiko FUKUSHIMA*, Nobuo UETA*, Hiroshi NAGANO**,1 and Ryoiti SHUKUYA**

*Department of Biochemistry, Teikyo University School of Medicine Kaga, Itabashi-ku Tokyo 173
**Department of Biochemistry, Nippon Medical School Sendagi, Bunkyo-ku, Tokyo 113

The chemical and physical properties of bullfrog serum low density lipoprotein (LDL) were investigated. On a weight percentage basis, LDL contained cholesterol ester, 30 3%; cholesterol, 5.6%; triglyceride, 12.5%; phospholipids, 23.3%; and protein, 22.4%.

The fatty acid compositions of triglycerides and major phospholipids from the bullfrog serum LDL were quite similar to those of human serum LDL. However, the fatty acid composition of the chlesterol ester from the bullfrog serum LDL was quite different from that of the human serum LDL.

Th average particle weight, determined by gel filtration, was 2 x 106 daltons. This value is very close to that of human LDL. In the fluorescence emission spectrum of bullfrog serum LDL, the emission maximum was 324 nm.

The amino acid composition of the apo-LDL resembled that of human apo-LDL.

1Present address Department of Physiological Chemistry and Nutrition, Faculty of Medicine, The University of Tokyo, Hongo, Bunkyoku, Tokyo 113.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.