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J. Biochem, 1977, Vol. 81, No. 5 1357-1365
© 1977 Japanese Biochemical Society

research-article

Properties of Proteins Produced after Damage to Deoxyribonucleic Acid of Escherichia coli1

Koji YODA, Kazuo NAGAI and Gakuzo TAMURA

Department of Agricultural Chemistry, University of Tokyo Bunkyo-ku, Tokyo 113

Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction), were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (approximately 40,000) and were produced under identical conditions, including requirement for the recA+ and lexA+ genotype. Sucrose density gradient centrifugation revealed that protein X' consisted of relatively large and heterogeneous aggregates in the cytoplasmic fraction; the distribution of protein X in the envelope was not determined. As proteins X and X' were shown to be equivalent, it is suggested that they are identical. Co-precipitation of the aggregates of protein X' with the envelope led to the appearance of protein X in the envelope fraction.

1This study was supported by a grant from the Ministry of Education, Science and Culture of Japan.


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