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J. Biochem, 1978, Vol. 83, No. 3 671-676
© 1978 Japanese Biochemical Society

research-article

Effect of Myosin DTNB Light Chain on the Actin-Myosin Interaction in the Presence of ATP

Tetsu HOZUMI and Ken HOTTA

Department of Physiology, Nagoya City University Medical School, Mizuho-ku, Nagoya Aichi 467

The influence of the DTNB light chain of myosin on its enzymatic activities was examined by studying the superprecipitation of actomyosin and the actin-activated ATPase of heavy meromyosin (HMM) [EC 3.6.1.3 [EC] ]. Although the Ca2+,Mg2+ and EDTA-ATPase activities of control and DTNB myosin were practically the same, the superprecipitation of actomyosin prepared from actin and DTNB myosin occurred more slowly than that of control myosin. The apparent binding constant obtained from double-reciprocal plots of actin-activated ATPase of DTNB HMM was lower than that of control HMM. Recombination of DTNB myosin and HMM with DTNB light chains restored the original properties of myosin and HMM. The removal of DTNB light chain from myosin had no effect on the formation of the rigor complex between actin and myosin. These results suggest that the DTNB light chain participates in the interaction of myosin with actin in the presence of ATP.


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