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J. Biochem, 1978, Vol. 83, No. 3 681-691
© 1978 Japanese Biochemical Society

research-article

Ergosterol Biosynthesis in Yeast

Pathways in the Late Stages and Their Variation under Various Conditions1

Takashi OSUMI2, Shigeru TAKETANI, Hirohiko KATSUKI3, Tomiko KUHARA* and Isamu MATSUMOTO*

Department of Chemistry, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606
*Research Laboratory of Gas Chromatography-Mass Spectrometry, Kurume University, School of Medicine Kurume, Fukuoka 830

3 To whom correspondence and reprint request should be addressed.

[Methyl-14C]methionine was supplied to yeast cells under aerobic and anaerobic conditions for the investigation of the pathway for ergosterol biosynthesis after the methylation of the side-chain. Under aerobic conditions, the incorporation of radioactivity into ergosterol was high. With a limited oxygen supply, in contrast, the radioactivity was first accumulated in ergosta-7, 24(28)-dien-3ß-ol and ergosta-8, 24(28)-dien-3ß-ol, and then transferred to ergost-7-en-3ß-oI, ergost-8-en-3ß-ol and ergosta-7, 22-dien-3ß-ol with time. Under strictly anaerobic conditions, a double bond was introduced neither to {Delta}5 nor to {Delta}22. The results of the tracer experiments suggested the operation of several pathways in the late stages of ergosterol biosynthesis. It was also suggested that the main pathways varied depending on the conditions such as oxygen supply and other factors. The above conclusion was supported by the results of the analyses of the sterol compositions of the cells grown under various conditions.

1 This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, No. 058037 and Ajinomoto Co., Inc.

2Present address: Department of Biochemistry, Faculty of Medicine, Shinshu University, Matsumoto, Nagano 390.


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