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J. Biochem, 1978, Vol. 83, No. 3 711-718
© 1978 Japanese Biochemical Society

research-article

A Compound Possessing Antitumor and Interferon-Inducing Acticites Derived from the Common Antigen (OEP) of Pseudomonas aeruginosa1

Ken-ich TANAMOTO*, Chiyoji ABE*, Yuzuru HOMMA*, Kazuo KURETANT**, Akio HOSHI** and Yasuhiko KOJIMA***

*Department of Bacteriology, Institute of Medical science, University of Tokyo Minato-Ku, Tokyo 108
**Pharmacology Division, National Cancer Center Research Institute Chuo-Ku, Tokyo 104
***Kitasato Institute Minato-Ku, Tokyo 108

OEP, a component consisting mainly of protein with small amounts of lipids and sugars, has been isolated from the autolysate of Pseudomonas aeruginosa and purified by phusico chemical methods. It possesses remarkable biological aproperties, showing antitumor and interferone-inducing activities.

As regards the antitumor activity of the sample, the ED50 value against ascites sarcoma-180 was I µg/kg mouse/ day, and its interfcron-inducing activity amounted to 15 units at a concentration of 0.01 µg/ml. Both acticities increased after protease digestion, reaching about ten times those of the sample which had not undergone digestion.

The protease-treated OEP contained 17% protein, 14.5% total sugars, 31% lipids, 12.5% hexosamine, 3.8% KDO, and 2.7% phosphorus. Neutral sugars consisted of 12.4% rhamnose, 2.7% mannose, 66.9% glucose, and other unidentigied material.

Total lipids derived from OEP consisted of 65% loosely-bound and 35% covalently-bound lipids: the former contained C14: 0, C15: 0, C16: 1, C18: 0, and C18:1 acids and the latter, ß-OH C10: 0, C13: 0, a-OH C12: 0, ß-OH C12: 0, C16: 0, and C16: 1 acids.

The antitumor and interferon-inducing activities of OEP remained after the removal loosely-bound lipids from OEP.

1 This work was supported by a grant-in-aid from the Japanese Ministry of Education, Science and Culture of Japan.


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