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J. Biochem, 1978, Vol. 83, No. 3 795-798
© 1978 Japanese Biochemical Society

research-article

Improved Preparation and Cooperative Calcium Contraction of Glycerinated Vorticella1

Hiroshi ASAI, Tsutomu OCHIAI, Keiji FUKUI, Masato WATANABE and Fumio KANO2

Department of Physics, Waseda University Nishi-okubo, Shinjuku-ku, Tokyo 160

An improved method for the preparation of glycerinated Vorticella convallaria was investigated. The pretreatment of living vorticellas with a medium containing 0.1% saponins and subsequent treatment with an extraction medium containing 35% glycerol at about 0°C was satisfactory. The equilibrium average length of contractile stalks of glycerinated vorticellas was measured at various free calcium concentrations in the reaction medium. It was found that the contractile element in the spasmoneme of the stalk is contracted by a cooperative interaction involving at least two calcium ions.

1 This work was aided in part by the Saneyoshi Science Foundation.

2Present address: Tokyo Metropolitan Laboratory of Public Health.


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