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J. Biochem, 1980, Vol. 87, No. 4 1105-1110
© 1980 Japanese Biochemical Society

research-article

Calorimetric Study of the Oxidation of Dithiothreitol

Harumi FUKADA and Katsutada TAKAHASHI

Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka Prefecture Sakai, Osaka 591

The enthalpy changes of the oxidation of dithiothreitol (DTT) by ferricyanide were measured calorimetrically in the pH range of 6 to 13 in various buffer solutions at 298 K. By combining the results of potentiometric titrations with the pH dependence of the observed enthalpy changes, the enthalpy changes of DTT oxidation and of the ionization of DTT sulfhydryls were evaluated. The enthalpy changes of oxidation of the non-ionized and the fully ionized DTT were found to be {Delta}HDTT (non-ionized) = 32±3 kJ mol–1 and {Delta}HDTT (fully ionized) = –38±3 kJ mol–1, respectively. The enthalpy changes of the first and the second ionizations of DTT sulfhydryls were found to be {Delta}H1 = 48±2 kJ mol–1 and {Delta}H2 = 21±3 kJ mol–1, respectively.


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