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J. Biochem, 1980, Vol. 87, No. 4 1167-1175
© 1980 Japanese Biochemical Society

research-article

Characterization of Two DNA Polymerases from Cauliflower Inflorescence1

Hirosuke FUKASAWA*, Masamitsu YAMAGUCHI**, Mei-Yin CHOU*, Hiroyuki MATSUMOTO* and Akio MATSUKAGE**

*Department of Biology, Faculty of Science, Kobe University Kobe, Hyogo 657
**Laboratory of Biochemistry, Aichi Cancer Center, Research Institute Chikusa-ku, Nagoya, Aichi 464

Two distinct DNA polymerases, A and B, isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea var. botrytis) inflorescence have been characterized, and compared with DNA polymerases, {alpha} and ß, from mouse myeloma. Polymerase-A bears a strong resemblance to polymerase-{alpha} from mammalian cells in all properties examined. The character of polymerase-B is also quite similar to polymerase-ß of mammalian cells in chromatographic elution properties, template-primer utilization, sensitivity to inhibitors, response to KCl or KP1 concentration, and other requirements for maximal activity, although it has a higher molecular weight (approx. 78,000) even in the presence of 0.25 M KC1 than polymerase-ß (mol. wt. less double equals50,000) of mammalian cells. This type of DNA polymerase has not been reported to exist in the plant system.

1 This study was supported in part by a grant (No. 448004) from the Ministry of Education, Science and Culture of Japan.


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