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J. Biochem, 1980, Vol. 87, No. 4 1209-1214
© 1980 Japanese Biochemical Society

research-article

Properties of Phosphorylated Myofibrils from Gizzard Smooth Muscle

Masahiro YAMAGUCHI* and Kazuko WATANABE**

*Department of Biochemistry and Nutrition, School of Physical Education, Juntendo University Narashino, Chiba 275
**Department of Biology, College of Science and Technology, Nihon University Funabashi, Chiba 274

Phosphorylation of chicken gizzard myosin light chain in myofibril and its effect on myofibrillar ATPase activity were investigated in the contracted state of myofibrils. When myofibrils were incubated for two hours at 30°C with ATP, magnesium and calcium, the myosin light chain was phosphorylated by endogenous light-chain kinasc. Standing overnight, the phosphorylated light chain was dephosphorylated by endogenous light-chain phosphatase. Control myofibril had much higher ATPase activity than phosphorylated and phosphorylated-dephosphorylated myofibrils. It was very interesting that the phosphorylated and phosphorylated-dephosphorylated myofibrils were quite similar in ATPase activity. However, phosphorylated myofibril differed from phosphorylated-dephosphorylated myofibril in Ca2+ dependency of Mg2+-ATPase activity. The phosphorylated-dephosphorylated myofibril was not affected by the presence or absence of Ca2+. In contrast, phosphorylated myofibril apparently showed a negative Ca2+-sensitivity. On the other hand, the results indicating that the superprecipitation gel formed by phosphorylated-dephosphorylated myosin could not be dissolved in 0.6 M NaCl, suggest that the phosphorylation-dephosphorylation process of the actomyosin system in gizzard myofibril results in stronger actin-myosin interaction.


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