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J. Biochem, 1980, Vol. 87, No. 4 1215-1219
© 1980 Japanese Biochemical Society

research-article

Analysis of the Acid-Base Titration Curve of Hen Lysozyme1

Seiki KURAMITSU and Kozo HAMAGUCHI

Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560

The acid-base titration curves of hen egg-white lysozyme [EC 3.2.1.17 [EC] ] obtained by three groups (Sakakibara & Hamaguchi (1968) J. Biochem. 64, 613–618; Tanford & Roxby (1972) Biochemistry 11, 2192–2198; Pfeil & Privalov (1976) Biophys. Chem. 4, 23–32) were analyzed using the empirical formula of Linderstrøm-Lang. Hen lysozyme has 32 ionizable groups including the {alpha}-amino and {alpha}-carboxyl groups. Of the 21 groups other than the 11 arginyl residues, the pK values of 17 ionizable groups have been determined by various methods. Using these pK values, the pK values of the other four ionizable groups were estimated. The apparent pK values obtained were 2.0 (pKint=3.4), 2.1 (pKint=3.5), 2.5 (pKint=3.8), and 7.9 (pKint=8.5) at 0.1 ionic strength and 25°C. The titration curves obtained by the above three groups were consistently explained in terms of the same set of pKint values. The results obtained also showed that no buried and untitratable groups are present in the native lysozyme molecule.

1 This work was supported in part by a grant for scientific research from the Ministry of Education, Science and Culture of Japan.


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