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J. Biochem, 1980, Vol. 87, No. 4 1221-1227
© 1980 Japanese Biochemical Society

research-article

Immunochemical Studies of Lipids

VI. Reactions of Anti-Sulfatide Antibodies with Sulfatide in Liposomal and Myelin Membranes1

Kei-ichi UEMURA, Matsuko YUZAWA-WATANABE, Nobuko KITAZAWA and Tamotsu TAKETOMI

Department of Biochemistry, Institute of Adaptation Medicine, Shinshu University Matsumoto, Nagano 390

Antibodies against sulfatide (3-sulfogalactopyranosylceramide) were raised in rabbits by an intradermal injection of a mixture of sulfatide and bovine serum albumin in Freund's complete adjuvant followed by three to five booster injections in incomplete adjuvant. Complement fixation assays done with liposomes containing lecithin-cholesterol-hapten (1 : 0.75: 0.1, by mol) demonstrated the reactivity of the antibodies with sulfatide, but not with galacto-sylceramide. The antibody activity was found entirely in the IgM fraction after Sephadex G-200 gel filtration. Anti-sulfatide antibodies were purified with liposomes as the immuno-adsorbent. Sulfatide in artificial and natural membranes could react with these antibodies to cause complement-dependent release of a fluorogenic marker from liposomes and resealed myelin membrane vesicles.

1 This study was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.


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