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J. Biochem, 1982, Vol. 91, No. 1 117-123
© 1982 Japanese Biochemical Society

research-article

Biosynthesis of Sulfite Oxidase in Rat Liver—Presence of a Larger Precursor Form of the Enzyme in Cytosol

Hideyu ONO and Akio ITO

Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812

The time courses of in vivo incorporation of [3H]leucine into mitochondrial, microsomal, and cytosolic sulfite oxidases were examined and it was found that the cytosol contained a precursor pool of newly synthesized sulfite oxidase which was rapidly transported into mitochondria. At 10 min after the injection of the radioactive leucine, most of the radioactivity of cytosolic sulfite oxidase was associated with the precursor whose molecular size was about 3,000 daltons larger than the authentic sulfite oxidase subunit. We concluded that sulfite oxidase was synthesized as a larger precursor in the cytosol, and transported into mitochondria to be converted to the mature enzyme. The turnover rate of sulfite oxidase in rat liver was also determined by measuring the decay of enzyme radioactivity after the injection of [14C]leucine. Mitochondrial, microsomal, and cytosolic sulfite oxidase had an almost identical half-life of 3–4 days.


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