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J. Biochem, 1982, Vol. 91, No. 1 143-155
© 1982 Japanese Biochemical Society

research-article

Structural Study of the Sugar Chains of Human Lactoferrin: Finding of Four Novel Complex-Type Asparagine-Linked Sugar Chains1

Akira MATSUMOTO, Hideo YOSHIMA, Seiichi TAKASAKI and Akira KOBATA

Department of Biochemistry, Kobe University School of Medicine Chuo-ku, Kobe, Hyogo 650

Human lactoferrin contains 2 asparagine-linked sugar chains in 1 molecule. These sugar chains were released as oligosaccharides by hydrazinolysis from two lactoferrin samples of different races. The two oligosaccharide fractions gave exactly the same fractionation pattern upon paper electrophoresis and Bio-Gel P-4 column chromatography after sialidase digestion. A structural study of the oligosaccharides obtained from the two samples by sequential exoglycosidase digestion in combination with methylation analysis also gave the same results indicating that there is no racial difference both in quality and quantity of the sugar chain moiety of lactoferrin. In addition to the two acidic sugar chains, NeuAc{alpha}2->6Galß1->4GlcNAcß1-> 2Man{alpha}1->6(NeuAc{alpha}2->6Galß1->4GlcNAcß1->2Man{alpha}1->3)Manß1->4GlcNAcß1->4(Fuc{alpha}1->6)GlcNAc and Galß1-> 4(Fuc{alpha}{alpha}1-> 3)GlcNAcß1-> 2Man{alpha}1-> 6(NeuAca2->6Galß1->4GlcNAcß1-> 2Man{alpha}1-> 3)Manß1->4GlcNAcß1->4(Fuc{alpha}1->3) GlcNac, and two novel acidic sugar chains, Galß1->4(Fuc{alpha}1->3)GlcNAcß1->2Man{alpha}1->6(neuAc{alpha}2->6Galß1->4GlcNAcß1->3GlcNAcß1->2Man{alpha}1->3)Manß1->4GlcNAß1->4(Fac{alpha}1->6)GlcNAc and Galß1->4(Fuc{alpha}1->3)GlcNAß1->3Galß1->4GlcNAcß1->2Man{alpha}1->6(NeuAc{alpha}2->6Galß1->4GlcNAcß1->2Man{alpha}1->3)Manß1->4GlcNAcß1->4(Fuc{alpha}1->6)GlcNAc, were found to occur in human lactoferrin.

1 This work was supported in part by research grants from the Yamanouchi Foundation for Research on Metabolic Disorders, and the Scientific Research Funds of the Ministry of Education, Science and Culture of Japan.


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