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J. Biochem, 1982, Vol. 91, No. 1 233-246
© 1982 Japanese Biochemical Society

research-article

The Asparagine-Linked Sugar Chains of Plasma Membrane Glycoproteins of K-562 Human Leukaemic Cells: A Comparative Study with Human Erythrocytes1

Hideo YOSHIMA*, Nobuyuki SHIRAISHI**, Akira MATSUMOTO*, Sakan MAEDA**, Taketoshi SUGIYAMA** and Akira KOBATA*

*Department of Biochemistry, Kobe University School of Medicine Chuo-ku, Kobe, Hyogo 650
**Department of Pathology, Kobe University School of Medicine Chuo-ku, Kobe, Hyogo 650

The paper electrophoretic pattern of the oligosaccharides released from the plasma membranes of K-562 cells by hydrazinolysis was quite different from that of human erythrocyte membranes. Bio-Gel P-4 column chromatography in combination with sequential exoglycosidase digestion of the neutral oligosaccharide fractions revealed that all those from K-562 cells are of the high mannose type, while those from erythrocytes are of large complex type structures.

Studies of the acidic oligosaccharides indicated that none of those obtained from K-562 cells contained the ß-N-acetylglucosamine residue linked at the C-4 position of the ß-mannosyl residue of the trimannosyl core, which occurs in most of the asparagine-linked sugar chains of human erythrocytes. This indicates that the glucosaminyltransferase that forms the GlcNAcß1->4Manß1->4 group has not been expressed in K-562 cells.

1This study was supported in part by research grants from the Scientific Research Funds of the Ministry of Education, Science and Culture of Japan, and the Cancer Division, Public Health Bureau, the Ministry of Health and Welfare, Japan.


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