Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by TANADA, S.
Right arrow Articles by TAKEMURA, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TANADA, S.
Right arrow Articles by TAKEMURA, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1982, Vol. 91, No. 1 291-299
© 1982 Japanese Biochemical Society

research-article

Interaction of Aminoacyl-tRNA with Bacterial Elongation Factor Tu : GTP Complex: Effects of the Amino Group of Amino Acid Esterified to tRNA, the Amino Acid Side Chain, and tRNA Structure

Shozo TANADA1, Makoto KAWAKAMI2, Koji NISHIO and Shosuke TAKEMURA

Institute of Molecular Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya, Aichi 464

2 To whom correspondence should be addressed

The present investigation was undertaken to see to what extent the a-amino group of the amino acid, the side chain of the amino acid of aminoacyl-tRNA, and th tRNA structure are involved in determining the affinity of aminoacyl-tRNA for bacterial elongation factor Tu-GTP complex. Various aminoacyl-tRNAs, mis aminoacylated tRNAs, and formylated aminoacyl-tRNAs were prepared, and the dissociation constants of the ternary complexes of aminoacyl-tRNA with EF-Tu: GTP were determined by the RNase-resistance assay. The results indicated that the free {alpha}-amino group of the amino acids in aminoacyl-tRNA is strongly required for binding with EF-Tu: GTP. In this connection, the biological significance of formylation for Met-tRNAMetf species is discussed.

1 Present address: Laboratory of Biochemical Preparation, Mitsubishi-Kasei Institute of Life Sciences, Minamiooya, Machida, Tokyo 194


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.