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J. Biochem, 1982, Vol. 91, No. 1 301-310
© 1982 Japanese Biochemical Society

research-article

Localization of a Glycosphingolipid, Asialo GM1, in Rat Immunocytes1

Takashi MOMOI, Kumiko NAKAJIMA, Kooko SAKAKIBARA and Yoshitaka NAGAI

Department of Pathobiochemical Cell Research, Institute of Medical Science, The University of Tokyo Shirokanedai, Minato-ku, Tokyo 108

One of the TLMA (rat T-lymphocyte-macrophage-associated antigens) was charac terized as asialo GM1, Gal(ß, 1–3)GalNAc(ß, 1–4)Gal(ß, 1–4)Glc(ß 1–1)-Cer by two assay methods using monospecific anti-asialo GM1 antibody, immunocytotoxicity testing and immunostaining.

Nylon column-enriched T lymphocytes (non-adherent cells) were highly cyto lyzed by anti-asialo GM1 antiserum and complement as compared with adherent cells (non T cells). Forty % of peritoneal exudate macrophages and 20% of granulocytes were also cytolyzed by anti-asialo GM1 antiserum. On the other hand, 5% of thymic cells and 60–80% of lymph node T cells were immunostained by anti asialo GM1 antibody. These asialo GM1-positive cells in thymus were found to be localized in the cortico-medulla junction. About 20% of macrophages and 2)% of granulocytes were also immunostained by anti-asialo GM1 F (ab')2 fragment. These results strongly support the previous conclusion that TLMA can be identified as asialo GMI. Asialo GM1 was universally expressed on a certain population of macrophages of various tissues, but not on all of the population. Alveolar macrophages were also asialo GM1-positive.

1 This research was supported in part by a grant from the Ministry of Education, Science and Culture of Japan, and also by a Research Grant for Specific Diseases from the Ministry of Health and Welfare of Japan.


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