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J. Biochem, 1982, Vol. 91, No. 1 67-71
© 1982 Japanese Biochemical Society

research-article

Immunological Studies on Cathepsins B and H from Rat Liver1

Eiki KOMINAMI and Nobuhiko KATUNUMA

Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University Tokushima, Tokushima 770

The immunological properties of thiol proteinases from rat liver, cathepsins B, H, and L, were examined. Rabbit antisera were prepared against cathepsins B and H from rat liver. Anti-cathepsin B and H sera quantitatively precipitated only the corresponding enzyme protein. On immunodiffusion analysis, anti-cathepsin B serum reacted with cathepsin B, but not with cathepsin H or cathepsin L, and similarly anti-cathepsin H reacted only with cathepsin H, indicating that the three thiol proteinases are distinct. Immunoelectrophoresis using a mixture of anti-cathepsin B and anti-cathepsin H showed that the precipitin line with cathepsin B moved faster to the anode than that with cathepsin H. Immunological diffusion with antisera indicated that rat liver cathepsins B and H were immunologically identical with cathepsins B and H from rat kidney, lung, spleen, brain, and heart, but different from cathepsin B or H from human or rabbit liver.

1 This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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