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J. Biochem, 1982, Vol. 91, No. 4 1103-1110
© 1982 Japanese Biochemical Society

research-article

Synthesis of Various Phospholipids from 2-Acyl Lysophospholipids by Escherichia Coli Extract1

Hiroshi HOMMA and Shoshichi NOJIMA

Department of Health Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113

When 2-[14C]acyl lysophosphatidylethanolamine was incubated with the envelope fraction of E. coli in the presence of Mg2+ ion, phosphatidylethanolamine, acylphos-phatidylglycerol and free fatty acid were produced. When 2-[14C]acyl lysophos-phatidylglycerol was examined similarly, six phospholipids as well as free fatty acid were produced. These were dilysocardiolipin, lysocardiohpin, phosphatidylglycerol, cardiolipin, bis(monoacylglycero)phosphate and acylphosphatidylglycerol; they were identified by thin layer chromatography, acetolysis and mild alkaline hydrolysis.

Studies with an E. Coli mutant which is deficient in cardiolipin synthase showed that dilysocardiolipin, lysocardiolipin and cardiolipin were synthesized by cardiolipin synthase. Bis(monoacylglycero)phosphate as well as acylphosphatidylglycerol was produced by acylphosphatidylglycerol synthase.

While phosphatidylglycerol and cardiolipin were produced predominantly from 2-acyl lysophosphatidylglycerol, almost the same amounts of dilysocardiolipin, lysocardiolipin and bis(monoacylglycero)phosphate were produced from the 1-acyl and 2-acyl isomers. Metabolites of 2-[14C]acyl lysophosphatidic acid were also examined. Phosphatidic acid, acylphosphatidylglycerol, free fatty acid and monoglyceride were produced, together with a small amount of diglyceride.

1This work was supported in part by Grant-in-Aid (Nos. 56118006 and 56370034) for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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