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J. Biochem, 1982, Vol. 91, No. 4 1249-1256
© 1982 Japanese Biochemical Society

research-article

Effect of Troponin and Tropomyosin on the Interaction between Cytochalasin B and Actin Filaments1

Noriko SUENAGA and Iwao OHTSUKI

Department of Pharmacology, Faculty of Medicine, Kyushu University Higashi-ku, Fukuoka, Fukuoka 812

Effects of troponin (TN) components and tropomyosin (TM) on the interactions between actin filament and cytochalasin B (CB) at low ionic strength were investigated using electron microscopy and viscometry. CB decreased the viscosity of the actin filament and this effect became apparent at 10 µM CB and reached the maximum at 40µm. CB (40 µM) shortened the average actin filament length from 0.50 to 0.25 µm as observed electron microscopically. Presence of TN components and TM diminished the effect of CB on both viscosity and filament length. TN-T but not TN-I had an inhibitory effect on the decrease of the viscosity of the A + TM filament induced by CB. TN-T1 but not TN-T2, one of two chymotryptic subfrag-ments of TN-T, had the same inhibitory effect seen with TN-T. Thus, the cooperation between the T1 region of TN-T and TM is probably essential for the inhibitory action on CB-actin interactions.

1This work was supported in part by research grants from the Muscular Dystrophy Association, Inc., the Ministry of Education, Science and Culture of Japan, and the Ministry of Health and Welfare of Japan.


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