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J. Biochem, 1982, Vol. 91, No. 6 1995-1998
© 1982 Japanese Biochemical Society


research-article

Study on the Interaction between Hemoglobin Izu (Macaca) and Organic Phosphates by Spin Labeling

Shin-ichi NAKAYAMA*, Masamatsu HOSHINO*, Osamu TAKENAKA**, Kenji TAKAHASHI**, Tokuko WATANABE*** and Masayuki YOSHIDA***

*Department of Chemistry, Faculty of Science, Saitama University Urawa, Saitama 338
**Department of Biochemistry, Primate Research Institute, Kyoto University Inuyama, Aichi 484
***Department of Chemistry, Faculty of Science, The University of Tokyo Bunkyo-ku, Tokyo 113

ESR spectra of the carbonmonoxy, oxy, and deoxy derivatives of hemoglobin Izu [Hb Izu (Macaca): ß 83 (EF 7) Gly->Cys] labeled at cysteine ß 83 with maleimide spin label have been observed in the presence and absence of 2,3-diphosphoglycerate and inositol hexaphosphate. The rc values obtained from the spectra indicated that inositol hexaphosphate binds to all the derivatives of Hb Izu, but 2,3-diphosphoglycerate only to the deoxy derivatives.


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