Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by NAKATSU, Y.
Right arrow Articles by SEKIGUCHI, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by NAKATSU, Y.
Right arrow Articles by SEKIGUCHI, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1982, Vol. 91, No. 6 2057-2065
© 1982 Japanese Biochemical Society

research-article

Action of T4 Endonuclease V on Polydeoxyribonucleotides with Apyrimidinic or Apurinic Sites1

Yoshimichi NAKATSU, Yusaku NAKABEPPU and Mutsuo SEKIGUCHI

Department of Biology, Faculty of Science, Kyushu University 33 Higasbi-ku, Fukuoka, Fukuoka 812

[3H]Thymine-labeled poly(dT) carrying many apyrimidinic (AP) sites has been prepared by treating an enzymatically synthesized poly(dA) poly(dT, dU) with uracil-DNA glycosylase. Incubation of the polymer with a homogeneous preparation of T4 endonuclease V resulted in conversion of the labeled material into acid-soluble forms. Native DNA with apurinic sites was also cleaved by the enzyme. Single-stranded polymers, poly(dT) carrying AP sites or poly(dT) with thymine dimers, were barely attacked by T4 endonuclease V. The polymer whose aldehyde moieties at AP sites were reduced to alcoholic forms was not susceptible to the enzyme. The site of endonucleolytic cleavage was determined by using alternating copolymers whose phosphate groups were differentially labeled. The result is consistent with the view that T4 endonuclease V cleaves a phosphodiester linkage on the 3'-side of AP sites, producing chains terminated at their 3'-ends with base-free deoxyribose and at their 5'-ends with phosphate.

1This work was supported by Scientific Research and Cancer Research Grants from the Ministry of Education, Science and Culture of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.