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J. Biochem, 1982, Vol. 91, No. 6 2091-2094
© 1982 Japanese Biochemical Society

other

The Inhibitory Mode of Aphidicolin on DNA Synthesis in NaCl-Treated HeLa Cell Nuclei1

Mieko OGURO and Hiroshi NAGANO

Department of Physiological Chemistry andiNutrition, Faculty of Medicine, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113

Isolated HeLa cell nuclei were treated with NaCl at various concentrations and inhibition by aphidicolin of DNA synthesis in the treated nuclei was studied. The inhibition was either noncompetitive or of the mixed type with respect to each dNTP when the nuclei were treated with NaCl at concentrations lower than 0.08 m. However, aphidicolin was a competitive inhibitor with respect to dCTP and a non-competitive or mixed type inhibitor with respect to the other 3 dNTPs when they were treated with NaCl at concentrations higher than 0.1 m. These results suggest the presence of nuclear factors) responsible for the changes in the inhibitory mode of aphidicolin on endogenous nuclear DNA synthesis.

1This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.


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