J. Biochem, 1982, Vol. 91, No. 6 2095-2098
© 1982 Japanese Biochemical Society
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Incorporation of a Ganglioside and a Spin-Labeled Ganglioside Analogue into Cell and Liposomal Membranes1
*Department of Health Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113
**School of Pharmaceutical Sciences, Showa University Hatanodai, Shinagawa-ku, 142
***Department of Biochemistry, School of Medicine, University of Marburg Marburg, Fed. Rep. of Germany
When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state was transferred to the lipid phase of the acceptor membranes. The transfer was temperature- and time-dependent, irrespective of the acceptor membranes, indicating that the rate of transfer is determined by the escape of monomers from the micelles.
The kinetics and temperature-dependence of the association of ganglioside II3NeuAc-GgOse4Cer with sheep erythrocytes was very similar to that of the "two tail" gangliosidoid, indicating that parts of ganglioside II3NeuAc-GgOse4Cer could be incorporated into the lipid phase of membranes via a similar mechanism.
This work was supported in part by Grants-in-Aid (Nos. 56370034, 56120010, and 00437017) for Scientific Research from the Ministry of Education, Science and Culture of Japan, the Deutsche Forschungsgemeinschaft (SFB 103-C4) and the Fond der Chemischen Industrie.