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J. Biochem, 1982, Vol. 92, No. 3 647-652
© 1982 Japanese Biochemical Society

research-article

Cytoplasmic Tubulin from Squid Nerve Fully Retains C-Terminal Tyrosine1

Takaaki KOBAYASHI* and Gen MATSUMOTO**

*Department of Biochemistry, The Jikei University School of Medicine Nishi Shinbashi, Minato-ku, Tokyo 105
**Electrotechnical Laboratory, Optoelectronics Section Tsukuba Science and Education City, Niihari-gun, Ibaraki 305

Cytoplasmic tubulin from squid nerve fully retains C-terminal tyrosine as a result of the almost zero activity of detyrosinating enzyme and low but definite activity of tubulin-tyrosine ligase in its cytoplasm, while the tyrosine content of cytoplasmic tubulin from mammalian brain is about 30 to 60% of that expected for the fully tyrosinated state because of much higher activity of detyrosinating enzyme in mammalian brain. On the other hand, membrane tubulin from both squid optic ganglion and mammalian brain retains only a small amount of C-terminal tyrosine. The significance of these differences is discussed.

1 This work was supported in part by a Grang-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 534032)


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