J. Biochem, 1983, Vol. 94, No. 1 199-205
© 1983 Japanese Biochemical Society
research-article |
Increased Thermal Stability of Collagen in the Presence of Sugars and Polyols1
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University Chikusa-ku, Nagoya, Aichi 464
The effects of sugars and polyols on the thermal denaturation temperature, Tm, of acid-soluble collagen from calf skin weTe studied at pH 4.0 under atmospheric pressure as well as high pressures up to 4,000 atm. Addition of these compounds invariably raised Tm with increases in their concentration over the whole range of pressure. The extent of stabilization by different sugars and polyols is discussed in terms of their different influences on the structure of water. The hydroxymethyl chain length of polyols and equatorial OH groups of the sugars were found to be decisive factors for their stabilizing effect on collagen structure. The similarity in their stabilizing effects on collagen and globular proteins suggests that our stabilization mechanism proposed for globular proteins can be essentially extended to fibrous proteins: such protein stabilization would be dominantly mediated through a preferential hydration of protein, originating in the water-structure-making character of sugars and polyols.
1 This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (57560119) and from the Towa Foods Society for Promotion of Research.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Mizuno, T. Hayashi, and H. P. Bachinger Hydroxylation-induced Stabilization of the Collagen Triple Helix: FURTHER CHARACTERIZATION OF PEPTIDES WITH 4(R)-HYDROXYPROLINE IN THE Xaa POSITION J. Biol. Chem., August 22, 2003; 278(34): 32373 - 32379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Devlin, H. Parfrey, D. J. Tew, D. A. Lomas, and S. P. Bottomley Prevention of Polymerization of M and Z alpha 1-Antitrypsin (alpha 1-AT) with Trimethylamine N-Oxide . Implications for the Treatment of alpha 1-AT Deficiency Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 727 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Goodson and K. D. Sarge Heat-inducible DNA Binding of Purified Heat Shock Transcription Factor 1 J. Biol. Chem., February 10, 1995; 270(6): 2447 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Bann and H. P. Bachinger Glycosylation/Hydroxylation-induced Stabilization of the Collagen Triple Helix. 4-trans-HYDROXYPROLINE IN THE Xaa POSITION CAN STABILIZE THE TRIPLE HELIX J. Biol. Chem., August 4, 2000; 275(32): 24466 - 24469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Beck, V. C. Chan, N. Shenoy, A. Kirkpatrick, J. A. M. Ramshaw, and B. Brodsky Destabilization of osteogenesis imperfecta collagen-like model peptides correlates with the identity of the residue replacing glycine PNAS, April 11, 2000; 97(8): 4273 - 4278. [Abstract] [Full Text] [PDF]
|
|