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J. Biochem, 1983, Vol. 94, No. 1 37-41
© 1983 Japanese Biochemical Society

research-article

Physicochemical and Immunochemical Properties of a Thermo-Labile Antigen (TLA a) from Yeast Cell Surface

Youichi TAMAI, Hiroshi SHINMOTO and Masayoshi TAKAKUWA

Department of Agricultural Chemistry, Faculty of Agriculture, Ehime University Matsuyama, Ehime 790

The physicochemical and immunochemical properties of a thermo-labile antigen (TLA a) which is located on the cell surface of Saccharomyces cerevisiae were studied. The sedimentation constant (s20,w) and molecular weight (sedimentation equilibrium method) were 6.26S and 68,800, respectively. The circular dichroic (CD) spectrum of TLA a had negative maxima at 210 and 221 nm, indicating the presence of {alpha}-structure of a polypeptide chain. The molar ratio of antibody to antigen which gave maximum precipitation was 2.7. Approximately 50% of the antigenic activity of heat-denatured TLA a was recovered when denatured molecules were dissolved in 6 M guanidine hydrochloride followed by 25-fold dilution with H2O. The amount of TLA a existing on the yeast cell surface was estimated to be 37.5µg per 10.5 mg of fresh cells, corresponding to 0.36% by weight of the fresh yeast.


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