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J. Biochem, 1984, Vol. 95, No. 6 1543-1549
© 1984 Japanese Biochemical Society

research-article

Genetically Regulated Expression of UDP-N-Acetylgalactosamine: GM3(NeuGc) N-Acetylgalactosaminyltransferase [EC 2.4.1.92] Activity in Mouse Liver1

Yasuhiro HASHIMOTO*, Mikiko ABE*, Yoshihiro KIUCHI**, Akemi SUZUKI* and Tamio YAMAKAWA*

*Metabolism Section, Tokyo Metropolitan Institute of Medical Science Honkomagome, Bunkyo-ku, Tokyo 113
**Laboratory Animal Science Section, Tokyo Metropolitan Institute of Medical Science Honkomagome, Bunkyo-ku, Tokyo 113

GM2 containing NeuGc was a major ganglioside in the liver of mouse strains such as BALB/c, DBA/2, C3H/He, and C57BL/10, whereas WHT/Ht mouse liver did not contain GM2(NeuGc) but contained GM3(NeuGc) as a major ganglioside. Since GM3(NeuGc) is a biosynthetic precursor of GM2(NeuGc), WHT/Ht liver was considered to lack the ability to synthesize GM2(NeuGc) from GM3(NeuGc) (Hashimoto, Y., et al. (1983) J. Biochem. 93, 895–901). In this study we measured the activity of UDP-N-acetylgalactosamine : GM3(NeuGc) N-acetylgalactosaminyltransferase in the liver of BALB/c, WHT/Ht, and their progeny. The transferase activity in the microsomal fraction of BALB/c liver was 2.10±0.32 x 10–5 units/mg protein (mean ± S.D.), whereas no activity was detected in that of WHT/Ht liver. F2 hybrids between BALB/c and WHT/Ht expressed GM2(NeuGc) as well as the enzyme activity, the level of which was almost half that in BALB/c liver (1.10± 0.12 x 10–5 units/mg protein). The backcross generation of F1 to WHT/Ht segregated into two groups with respect to expression of GM2(NeuGc) and the transferase activity: 11 of the 21 mice analyzed expressed both GM2(NeuGc) and the transferase activity (1.28 ± 0.18 x 10–5 units/mg protein), whereas the rest expressed neither. These results suggest that the expression of GM2(NeuGc) is directly regulated by the activity of UDP-N-acetylgalactosamine : GM3(NeuGc) N-acetylgalactosaminyltransferase in mouse liver.

1 This study was supported in part by grants from the Ministry of Education, Science and Culture of Japan, the Science and Technology Agency of Japan and the Foundation for the Promotion of Research on Medical Resources.


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