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J. Biochem, 1984, Vol. 95, No. 6 1551-1557
© 1984 Japanese Biochemical Society

research-article

Properties of Calcium-Dependent Regulatory Proteins from Fungi and Yeast

Taro NAKAMURA*,1, Kenichi FUJITA*, Yoshitomo EGUCHI* and Michio YAZAWA**

*Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University Kita-ku, Sapporo, Hokkaido 060
**Department of Chemistry, Faculty of Science, Hokkaido University Kita-ku, Sapporo, Hokkaido 060

2To whom requests for reprints should be addressed

Calmodulins were isolated from vegetative mycelia of Basidiomycetes fungi, Agaricus campestris and Coprinus lagopus. These calmodulins showed similar mobilities to those of animal calmodulins on nondenaturing polyacrylamide gel electrophoresis in the presence or absence of Ca2+. The molecular weights of both calmodulins were determined to be 16,000. Agaricus calmodulin consisted of 148 amino acids including {varepsilon}-N-trimethyllysine and cysteine. The UV-absorption spectrum showed the relatively high content of phenylalanine in Basidiomycetes calmodulins. The difference UV-absorption spectrum due to the blue shift by Ca2+ was observed. Both calmodulins activated muscle myosin light chain kinase and pea NAD+ kinase in a Ca2+-dependent manner, and the activities were inhibited by trifluoperazine or chlorpromazine.

A calmodulin-like protein was partially purified from baker's yeast, Saccharomyces cerevisiae. However, detection of a calmodulin-like protein in prokaryotes was not successful.


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