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J. Biochem, 1984, Vol. 95, No. 6 1655-1659
© 1984 Japanese Biochemical Society

research-article

Human Serum Contains N-Acetyflactosamine : ß1–3 N-Acetylglucosaminyltransferase Activity

Osamu HOSOMI, Akira TAKEYA and Tadahisa KOGURE

Department of Legal Medicine, School of Medicine, Gunma University Maebashi, Gunma 371

Human serum was shown to contain N-acetyllactosamine: N-acetylglucosaminyltransferase activity. The reaction product was hydrolyzed by ß-N-acetylglucosaminidase and released [14C]N-acetylglucosamine, indicating that the N-acetylglucosaminyl residue was ßlinked to N-acetyllactosamine. Methylation and hydrolysis of the reaction product yielded 2,4,6-trimethyl[3H]galactose, indicating that the N-acetylglucosaminyl residue was introduced at position C-3 of the terminal galactose of N-acetyllactosamine. In our experiments, 2,3,4-trimethyl[3H]galactose was not detected. Substrate competition studies between N-acetyllactosamine and lactose showed that this enzyme also catalyzed the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to lactose. Since the Km value for N-acetyllactosamine, which was 7.0 mM, was approximately a fourth of that for lactose (29.8 mM), N-acetyllactosamine was more effective than lactose as an acceptor.


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