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J. Biochem, 1984, Vol. 95, No. 6 1823-1826
© 1984 Japanese Biochemical Society

research-article

Antibody against 25,000 Dalton Tryptic Fragment of Subfragment-1 from Chicken Skeletal Muscle Myosin: Functional Implication of the 25,000 Fragment Region in Subfragment-11

Takayuki MIYANISHI*, Takashi SAKU** and Genji MATSUDA*,2

*Department of Biochemistry, Nagasaki University School of Medicine Sakamotomachi, Nagasaki, Nagasaki 852
**Department of Oral Pathology, Nagasaki University School of Dentistry Sakamotomachi, Nagasaki, Nagasaki 852

2To whom correspondence should be addressed

Antibody was prepared against the 25,000-dalton tryptic fragment of subfragment-1 from skeletal muscle myosin. The antibody was found to inhibit the Mg2+-ATPase activity and the initial P1-burst of the ATPase. The antibody suppressed the ATP induced fluorescence enhancement of S-1, though it did not suppress the binding of ATP to S-1. The acto-S-1 ATPase activity was also inhibited by the antibody. These results suggest that there is a site in the 25K fragment region responsible for the transition of the myosin-ATP complex to another high energy complex.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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