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J. Biochem, 1984, Vol. 95, No. 6 1827-1830
© 1984 Japanese Biochemical Society

research-article

Immunological Cross-Reactivity between Large and Small (2'-5')Oligoadenylate Synthetases from Pig Cells

Noriaki SHIMIZU*,1, Yoshihiro SOKAWA*,2 and Junko SOKAWA**

*Institute for Virus Research, Kyoto University Sakyo-ku, Kyoto, Kyoto 606
**Radioisotope Research Center, Kansai Medical University Moriguchi, Osaka 570

2To whom correspondence should be addressed

Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2–5A synthetases) were estimated. The 2–5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20–40 kDa. The nuclear 2–5A synthetase was selectively adsorbed to Protein A-Sepharose beads conjugated with anti-spleen 2–5A synthetase antibody. Thus, the smaller 2–5A synthetase in nuclei of pig cells shares a protein structure with the larger enzyme from pig spleen.

1Present address: Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Azusawa, Itabashi-ku, Tokyo 174.


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