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J. Biochem, 1984, Vol. 96, No. 4 1257-1265
© 1984 Japanese Biochemical Society

research-article

Glycolipid Composition of Ascitic Fluids from Patients with Cancer1

Takao TAKI*, Yasuo KOJIMA*, Hiroyuki SETO*, Hideo YAMADA** and Makoto MATSUMOTO*,2

*Department of Biochemistry, Shizuoka College of Pharmacy Oshika, Shizuoka 422
**Department of Nuclear Medicine and Radiology, Tokyo Metropolitan Geriatric Hospital Sakae-cho, Itabashi-ku, Tokyo 173

2To whom all correspondence should be addressed.

The glycolipid composition of ascitic fluids from nine patients with cancer and one pleural effusion from a hepatoma patient was studied. Glucosylceramide, lactosylceramide, globotriaosylceramide, and globotetraosylceramide were found in all samples and also in normal human serum. These glycolipids accounted for more than 90% of the neutral glycolipid fraction and the composition in ascitic fluids was similar to that in normal human serum. From ascitic fluids, several minor grycolipids, which could not be detected in normal human serum, were isolated and characterized by exoglycosidase treatment. Lactoneotetraosylceramide was found in eight samples of ascitic fluids, and globopentaosylceramide was detected in two samples from hepatoma and one from pancreatic cancer. A fucolipid which was converted to lactoneotetraosylceramide by {alpha}-L-fucosidase treatment was recognized in two samples from hepatoma patients. In the ganglioside fraction, GM, was the predominant component both in normal human serum and in ascitic fluid. The GM2 content in ascitic fluids was much higher than that in normal human serum. From these results, lactoneotetraosylceramide and GM2 are possible candidates as cancer markers, because they seemed to be derived from cancer tissues by shedding.

1This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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