Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by YAMANOHA, B.
Right arrow Articles by YASUHARA, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by YAMANOHA, B.
Right arrow Articles by YASUHARA, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1984, Vol. 96, No. 4 1273-1281
© 1984 Japanese Biochemical Society

research-article

Differences between Homogeneous Spermidine Synthases Isolated from Rat and Pig Liver1

Banri YAMANOHA*, Keijiro SAMEJIMA*,2, Terumi NAKAJIMA** and Tadashi YASUHARA**

*Tokyo Biochemical Research Institute Takada, Toshima-ku, Tokyo 171
**Institute for Medical and Dental Engineering, Tokyo Medical and Dental University Kandasurugadai, Chiyoda-ku, Tokyo 101

Spermidine synthase was purified to homogeneity from rat and pig liver by a method modified from a previously reported one using DEAE-Sepharose, S-adenosyl(5')-3-thiopropylamine-Sepharose affinity chromatography, Sephacryl S-300 gel filtration and polyacrylamide gel electrophoresis. No apparent difference between the two enzymes was observed in specific activity, molecular weight (74,000), or subunit composition (two subunits). However, significant differences were observed in their pI values, which were 5.16 for the pig enzyme and 5.34 for the rat enzyme, and their peptide maps. Amino acid compositions of the two enzymes were closely related, but differed significantly in some amino acids. In addition, the rat enzyme was more sensitive to inhibition by S-adenosyl-1, 8-diamino-3-thiooctane than the pig enzyme.

2Present address: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado-shi, Saitama 350–02. To whom correspondence should be addressed.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.