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J. Biochem, 1984, Vol. 96, No. 5 1409-1418
© 1984 Japanese Biochemical Society

research-article

Serratia Protease. Amino Acid Sequences of Both Termini, the 53 Residues in the Middle Region Containing the Sole Methionine Residue, and a Probable Zinc-Binding Region1

In Sook LEE*, Sadao WAKABAYASHI*, Kouichi MIYATA**, Katsumi TOMODA**, Masahiko YONEDA**, Kenji KANGAWA***, Naoto MINAMINO****, Hisayuki MATSUO*** and Hiroshi MATSUBARA*

*Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560
**Central Division, Takeda Chemical Industries, Ltd., Yodogawa-ku Osaka, Osaka 532
***Department of Biochemistry Kiyotake, Miyazaki 889-16
****Department of Anesthesiology, Miyazaki Medical College Kiyotake, Miyazaki 889-16

Reexamination of the molecular mass and the amino acid composition of Serratia protease revealed the presence of 1 mol of methionine per mol of protein (about 46K daltons), and this was confirmed by BrCN cleavage followed by separation of the two fragments. The sole methionine residue was located near the middle region of the molecule. The amino(N)-terminal sequence was determined by Edman degradation of the protein and studies of several proteolytic peptides, establishing a sequence of 18 residues with a heterogeneous N-terminus. The carboxyl(C)-terminal sequence was determined by carboxypeptidase A digestion and tritium-labeling of the citraconylated C-terminal half segment to be -Phe-Ile-Val. The sequences of a total of 53 residues containing the methionine residue and a total of 38 residues containing two histidine residues were established by the application of various conventional methods to a BrCN peptide and several proteolytic peptides. The segment containing the histidine residues was homologous with that containing the two histidine residues chelating the zinc atom of thermolysin. The 38-residue segment may be directly connected to the 53-residue segment.

1 A part of this work was presented at the 56th annual meeting of the Japanese Biochemical Society held in Fukuoka in 1983.


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