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J. Biochem, 1986, Vol. 99, No. 5 1455-1464
© 1986 Japanese Biochemical Society

research-article

Structure and Expression of a Cloned cDNA for Human (2'-5')Oligoadenylate Synthetase1

Satoshi SHIOJIRI, Rikiro FUKUNAGA, Yoshitake ICHII and Yoshihiro SOKAWA

Institute for virus Research, Kyoto University Sakyo-ku, Kyoto 606

17S po1y(A)+ which hybridized to an oligonucleotide complementary to a part of the partial cDNA (ElcDNA) (Merlin et al. (1983) Proc. Natl Acad. Sci. U.S. 80, 4904) for 2-5A synthetase, was isolated from interferon-treated human KB cells and used for cDNA cloning. Several overlapping cDNAs were cloned by using the oligonucleotide as a probe. Two of them were joined at their overlapping region, resulting in a cDNA (22-1cDNA) of 1.4kb containing a long open reading frame. When the cDNA was expressed in COS-7 cells with an eukaryotic promoter, active 2-5A synthetase was produced and localized mainly in the cytoplasm. The 5'-proximal ATG in 22-1 cDNA is followed immediately by another ATG. This second ATG was assumed to work as the initiator codon. If so, this enzyme com prises 363 amino acids.

1 This work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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