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J. Biochem, 1986, Vol. 99, No. 5 1525-1532
© 1986 Japanese Biochemical Society

research-article

The Small Subunits of Calcium Dependent Proteases with Different Calcium Sensitivities are Identical1

Hiroshi KAWASAKI*, Shinobu IMAJOH*, Seiichi KAWASHIMA**, Hiroaki HAYASHI*** and Koichi SUZUKI*

*Department of Molecular Biology, The Tokyo Metropolitan Institute of Medical Science 3–18–12, Honkomagome, Bunkyo-ku, Tokyo 113
**Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology Sakaecho, Itabashi-ku, Tokyo 173
***Institute of Endocrinology, Gunma University Maebashi, Gunma 371

The small subunits of two calcium dependent proteases from rabbit with different calcium sensitivities were isolated by high performance liquid chromatography (HPLC) and their properties were compared. The isolated subunits were indistinguishable on polyacrylamide gel electrophoresis and HPLC. Their amino acid compositions were identical, and the peptides obtained on their digestion with lysylendopeptidase showed identical peptide maps on HPLC. Furthermore, the amino acid compositions and partial amino acid sequences of the corresponding peptides purified by HPLC were the same. These results indicate that the two calcium protease isozymes possess the same small subunit.

1 This study was supported in part by research grants from the Ministry of Education, Science and Culture, a Grant-in-Aid for New Drug Development, and grants from the National Center for Nervous, Mental and Muscular Disorders from the Ministry of Health and Welfare of Japan.


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