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J. Biochem, 1986, Vol. 99, No. 5 1533-1535
© 1986 Japanese Biochemical Society

other

Conformations of Fibroblast and E. coli-Derived Recombinant Human Interferon-ßs as Studied by Nuclear Magnetic Resonance and Circular Dichroism

Jun UTSUMI*, Shojiro YAMAZAKI*, Kazuo HOSOI*, Hirohiko SHIMIZU*, Ken KAWAGUCHI** and Fuyuhiko INAGAKI**,1

*Basic Research Laboratories Toray Industries, Inc.
**Toray Research Center, Inc. Kamakura, Kanagawa 248

The conformations of fibroblast and E. coli-derived recombinant human interferonßs were studied by circular dichroism and nuclear magnetic resonance spectroscopy in the acidic pH region of 4.6 to 1.6. Both interferons have very similar conformations with high {alpha}-helix contents (~70%). These results suggest that glycosylation does not appreciably change the conformation of human interferon-ß. Moreover, a slow conformational change is observed below pH 2.0, which induces the disruption of ß-sheets.

1Present address: The Tokyo Metropolitan Institute of Medical Science, 18-22, Honkomagome 3-chome, Bunkyo-ku, Tokyo 113.


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